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不同诱导子对枇杷悬浮细胞生长及熊果酸、齐墩果酸含量的影响
引用本文:李惠华,刘小英,徐剑,常强,王伟,苏明华. 不同诱导子对枇杷悬浮细胞生长及熊果酸、齐墩果酸含量的影响[J]. 亚热带植物科学, 2014, 43(3): 187-191. DOI: 10.3969/j.issn.1009-7791.2014.03.001
作者姓名:李惠华  刘小英  徐剑  常强  王伟  苏明华
作者单位:(福建省亚热带植物研究所,福建省亚热带植物生理生化重点实验室,福建 厦门 361006)
基金项目:福建省自然科学基金(2012J05047);福建省公益类科研院所专项(2011R1012-1);厦门市科技计划项目(3502Z20132004)
摘    要:在已建立的稳定枇杷细胞悬浮培养系的试验中,研究加入诱导子水杨酸(SA)、茉莉酸甲酯(MJ)、水解酪蛋白(CH)和酵母提取物(YE)对枇杷悬浮细胞生长及次生代谢产物熊果酸(UA)、齐墩果酸(OA)含量的影响。结果表明,从细胞生长、UA及OA含量三方面综合考虑,以MJ为100.0 mg.L-1时培养最佳,此时细胞生长、UA及OA含量分别为对照的1.081、2.540及2.590倍。

关 键 词:枇杷  细胞悬浮培养  诱导子  熊果酸  齐墩果酸  
收稿时间:2014-05-04
修稿时间:2014-05-04

Influence of Different Elicitors on Cell Growth,Ursolic Acid and Oleanic Acid Content of Loquat Cell Suspension Culture
LI Hui-hua,LIU Xiao-ying,XU Jian,CHANG Qiang,WANG Wei,SU Ming-hua. Influence of Different Elicitors on Cell Growth,Ursolic Acid and Oleanic Acid Content of Loquat Cell Suspension Culture[J]. Subtropical Plant Science, 2014, 43(3): 187-191. DOI: 10.3969/j.issn.1009-7791.2014.03.001
Authors:LI Hui-hua  LIU Xiao-ying  XU Jian  CHANG Qiang  WANG Wei  SU Ming-hua
Affiliation:(Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China)
Abstract:Using the suitable loquat cell suspension culture as test materials, the influence of different elicitors (salicylic acid, SA; jasmonic acid, MJ; casein acid hydrolysate, CH; yeast extract, YE) on the cell growth, the accumulation of ursolic acid (UA) and Oleanic acid (OA) were studied. The results showed that the addition of MJ 100.0 mg.L-1 was advantageous to loquat cell suspension culture, the fresh weight, UA and OA content, were 1.081, 2.540, 2.590 times to the control, respectively.
Keywords:loquat   cell suspension   elicitor   ursolic acid   oleanic acid  
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