Inter-Ring Communication Is Dispensable in the Reaction Cycle of Group II Chaperonins |
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| Authors: | Yohei Y. Yamamoto Yuki Abe Kazuki Moriya Mayuno Arita Keiichi Noguchi Noriyuki Ishii Hiroshi Sekiguchi Yuji C. Sasaki Masafumi Yohda |
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| Affiliation: | 1 Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184–8588, Japan;2 Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305–8566, Japan;3 Japan Synchrotron Radiation Research Institute, Sayo, Hyogo 679–5198, Japan;4 CREST Sasaki Team, Japan Science and Technology Agency, Tokyo 102–0076, Japan;5 Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba 277–8561, Japan |
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| Abstract: | Chaperonins are ubiquitous molecular chaperones with the subunit molecular mass of 60 kDa. They exist as double-ring oligomers with central cavities. An ATP-dependent conformational change of the cavity induces the folding of an unfolded protein that is captured in the cavity. In the group I chaperonins, which are present in eubacteria and eukaryotic organelles, inter-ring communication takes important role for the reaction cycle. However, there has been limited study on the inter-ring communication in the group II chaperonins that exist in archaea and the eukaryotic cytosol. In this study, we have constructed the asymmetric ring complex of a group II chaperonin using circular permutated covalent mutants. Although one ring of the asymmetric ring complex lacks ATPase or ATP binding activity, the other wild-type ring undergoes an ATP-dependent conformational change and maintains protein-folding activity. The results clearly demonstrate that inter-ring communication is dispensable in the reaction cycle of group II chaperonins. |
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| Keywords: | DXT, diffracted X-ray tracking CS, citrate synthase Ni-NTA, nickel-nitrilotriacetic acid |
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