The 1.8-Å Crystal Structure of the N-Terminal Domain of an Archaeal MCM as a Right-Handed Filament |
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| Authors: | Yang Fu Ian M Slaymaker Junfeng Wang Ganggang Wang Xiaojiang S Chen |
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| Institution: | 1 Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA;2 Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA;3 Center of Excellence in NanoBiophysics, University of Southern California, Los Angeles, CA 90089, USA;4 Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90089, USA |
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| Abstract: | Mini-chromosome maintenance (MCM) proteins are the replicative helicase necessary for DNA replication in both eukarya and archaea. Most of archaea only have one MCM gene. Here, we report a 1.8-Å crystal structure of the N-terminal MCM from the archaeon Thermoplasma acidophilum (tapMCM). In the structure, the MCM N-terminus forms a right-handed filament that contains six subunits in each turn, with a diameter of 25 Å of the central channel opening. The inner surface is highly positively charged, indicating DNA binding. This filament structure with six subunits per turn may also suggests a potential role for an open-ring structure for hexameric MCM and dynamic conformational changes in initiation and elongation stages of DNA replication. |
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| Keywords: | MCM mini-chromosome maintenance dsDNA double-stranded DNA ssDNA single-stranded DNA EM electron microscopy |
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