| 真菌激发子对桦褐孔菌多酚积累的影响 |
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| 引用本文： | 赵艳霞,缪康杰,张梅梅,郑维发. 真菌激发子对桦褐孔菌多酚积累的影响[J]. 菌物学报, 2010, 29(3): 437-441 |
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| 作者姓名： | 赵艳霞 缪康杰 张梅梅 郑维发 |
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| 作者单位： | 徐州师范大学,江苏省药用植物生物技术重点实验室,徐州,221116 |
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| 基金项目： | 江苏省自然科学基金（No. BK2009084）；徐州师范大学国家自然科学基金预研究项目（No. 08XLY14） |
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| 摘 要： | 作者旨在阐明真菌激发子对桦褐孔菌多酚积累的影响。以摇瓶法培养桦褐孔菌,在其培养液中加入真菌激发子和一氧化氮合酶抑制剂氨基胍,观察桦褐孔菌多酚和一氧化氮的积累并测定菌丝体内一氧化氮合酶和苯丙氨酸解氨酶的活性。多酚以Folin-Ciocalteu法测定,一氧化氮的积累量用硝酸还原酶法测定,一氧化氮合酶和苯丙氨酸解氨酶活性均以分光光度计法测定。结果表明,添加45μg/mL的激发子可使桦褐孔菌菌丝体多酚的积累量达到46.5mg/g,显著地高于正常培养的菌丝体多酚积累量34.6mg/g;同时加入45μg/mL的激发子和10mmol/L氨基胍则使菌丝体多酚积累的最高水平降为34.8mg/g。此外,激发子的加入显著促进了一氧化氮的产生并提高了苯丙氨酸解氨酶活性,而这种促进和提升作用为氨基胍所抑制。这表明真菌激发子能显著地提升桦褐孔菌多酚类化合物的积累,而一氧化氮可能是这种提升作用的信号传导分子。
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| 关 键 词： | 深层培养 一氧化氮 一氧化氮合酶 苯丙氨酸解氨酶 |
Effects of fungal elicitor on production of phenolic compounds by Phaeoporus obliquus |
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| ZHAO Yan-Xi,MIAO Kang-Jie,ZHANG Mei-Mei and ZHENG Wei-Fa. Effects of fungal elicitor on production of phenolic compounds by Phaeoporus obliquus[J]. Mycosystema, 2010, 29(3): 437-441 |
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| Authors: | ZHAO Yan-Xi MIAO Kang-Jie ZHANG Mei-Mei ZHENG Wei-Fa |
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| Affiliation: | Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China |
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| Abstract: | This study was designed to evaluate the effects of fungal elicitor on the production of phenolic compounds in submerged cultures of Phaeoporus obliquus. Fungal elicitor and amino guanidine were supplemented to the cultures to determine the changes in accumulation of phenolic compounds and nitric oxide, the activities of nitric oxide synthase and phenylalanine ammonium-lyase. Phenol levels were measured using Folin-Ciocalteu method. Nitric oxide was measured by nitrate reductase method. The activities of nit... |
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| Keywords: | submerged cultures nitric oxide nitric oxide synthase phenylalanine ammonium-lyase |
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