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Fatal toxoplasmosis in a southern muriqui (Brachyteles arachnoides) from São Paulo state,Brazil: Pathological,immunohistochemical, and molecular characterization
Authors:Stéfanie Vanessa Santos  Hilda FJ Pena  Mauricio G Talebi  Rodrigo HF Teixeira  Cristina T Kanamura  Josué Diaz‐Delgado  Solange M Gennari  José Luiz Catão‐Dias
Institution:1. Laboratory of Wildlife Comparative Pathology, Department of Pathology, School of Veterinary Medicine, University of S?o Paulo, S?o Paulo, Brazil;2. Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of S?o Paulo, S?o Paulo, Brazil;3. Universidade Federal de S?o Paulo (UNIFESP) ‐ Campus Diadema, Diadema, S?o Paulo, Brazil;4. Parque Zoológico Municipal “Quinzinho de Barros” (PZMQB), Sorocaba, Brasil;5. Centro de Patologia, Instituto Adolfo Lutz (IAL), S?o Paulo, Brazil
Abstract:We report the pathological, immunohistochemical, and molecular features of fatal acute systemic toxoplasmosis in an adult, female, free‐living southern muriqui (Brachyteles arachnoides) from São Paulo state, Brazil. PCR‐RFLP genotyping analysis identified the #21 genotype of Toxoplasma gondii. This represents the first report of acute toxoplasmosis involving this genotype in humans and animals.
Keywords:conservation  genotyping  microsatellite markers  nonhuman primate  PCR‐RFLP     Toxoplasma gondii     wildlife
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