Characterization of a PRISTANE‐induced lupus‐associated model in the non‐human primate cynomolgus monkey |
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| Authors: | Jing Wang Hui Shen Yuqiang Zhu Ying Zhu Lei Cai Zhiyao Wang Qin Shi Yuhua Qiu |
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| Affiliation: | 1. Department of Immunology, Medical College, Soochow University, Jiangsu, China;2. Laboratory Animal Center, Soochow University, Jiangsu, China;3. Central Laboratory, The First hospital of Jiaxing, Zhejiang, China;4. Center for Clinical Laboratory, First Affiliated Hospital of Soochow University, Jiangsu, China;5. Department of Orthopedics, First Affiliated Hospital of Soochow University, Jiangsu, China |
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| Abstract: | Background Lupus is an autoimmune disease with complex syndrome. Rodent models have limitations for recapitulating the spectrum of the disease. A more powerful translational model is desirable. Method Lupus‐associated model in cynomolgus monkeys was induced by two intraperitoneal injections of 2, 6, 10, 14‐tetramethylpentadecane (PRISTANE). Lupus‐specific biomarkers and manifestations over a 246‐day period were observed at multilevel. To visualize and quantify kidney function in real time, contrast‐enhanced ultrasound was used. Results The indicative biomarkers and manifestations fulfilled major diagnosis criteria according to the “Criteria of Lupus” of the American College of Rheumatology. Significant changes in time‐intensity curve parameters were observed, indicating impaired renal function and the method as a feasible, non‐invasive diagnostic method in primate model. Conclusions We successfully induced lupus‐associated model with systemic lupus syndrome. This primate model can be a valuable translational model for further pathogenesis and symptomology studies and for exploring therapeutic candidates. |
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| Keywords: | animal model contrast‐enhanced ultrasound cynomolgus monkey PRISTANE systemic lupus erythematosus |
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