Arabidopsis thaliana NOP10 is required for gametophyte formation |
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| Authors: | Lin‐Xiao Li Hong‐Ze Liao Li‐Xi Jiang Qing Tan De Ye Xue‐Qin Zhang |
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| Affiliation: | 1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China;2. State Key Laboratory of Non‐Food Biomass Energy and Enzyme Technology, National Engineering Research Center for Non‐Food Biorefinery and Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China;3. College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | The female gametophyte is crucial for sexual reproduction of higher plants, yet little is known about the molecular mechanisms underlying its development. Here,we report that Arabidopsis thaliana NOP10(AtNOP10) is required for female gametophyte formation. AtNOP10 was expressed predominantly in the seedling and reproductive tissues, including anthers, pollen grains, and ovules.Mutations in AtNOP10 interrupted mitosis of the functional megaspore during early development and prevented polar nuclear fusion in the embryo sacs. AtNOP10 shares a high level of amino acid sequence similarity with Saccharomyces cerevisiae(yeast) NOP10(ScNOP10), an important component of the H/ACA small nucleolar ribonucleoprotein particles(H/ACA sno RNPs) implicated in 18 S r RNA synthesis and r RNA pseudouridylation. Heterologous expression of ScNOP10 complemented the mutant phenotype of Atnop10. Thus, AtNOP10 influences functional megaspore mitosis and polar nuclear fusion during gametophyte formation in Arabidopsis. |
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