Molecular weight determination of peptides by high-performance gel permeation chromatography |
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| Authors: | W O Richter B Jacob P Schwandt |
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| Affiliation: | Raymond Purves Research Laboratories (University of Sydney), Royal North Shore Hospital of Sydney, St. Leonards, New South Wales 2065 Australia |
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| Abstract: | A method for molecular weight determination of small peptides using Bio-Sil TSK 20 and Bio-Gel TSK 125 columns is described. The TSK 20 column provided a good separation of the standard peptides in the range from 1000-10,000 with an accuracy of less than 5% from the calculated regression line. Two combined TSK 125 columns allowed a reliable molecular weight determination in the range from 800 to 3500. |
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| Keywords: | molecular weight determination peptides HPLC guanidine hydrochloride |
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