Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I |
| |
| Authors: | Marc Jakoby Martin Tesch Hermann Sahm Reinhard Krämer reas Burkovski |
| |
| Institution: | Laboratoire ORSTOM de Microbiologie Anaérobies, Universitéde Provence, CESB-ESIL case 925, 163 avenue de Luminy, 13288 Marseille Cedex 9, France;Sanofi Recherche, Groupe Elf-Aquitaine, 31676 Labège Cedex, France;School of Biomolecular and Biomedical Sciences, Faculty of Science and Technology, Griffith University, Brisbane, Qld. 4111, Australia;Unitédes Entérobactéries, INSERM U199, Institut Pasteur, 75724 Paris Cedex 15, France;Département de Biologie, Universitéde la Méditerranée, 13288 Marseille Cedex 9, France |
| |
| Abstract: | The Corynebacterium glutamicum glutamine synthetase I (GSI) structural gene glnA was cloned by a PCR approach using oligonucleotide primers derived from conserved amino acid sequences of the GSI proteins from various bacteria. Disruption or deletion of this gene in C. glutamicum led to a glutamine auxotrophic phenotype and complete loss of glutamine synthetase activity, indicating the key role of this enzyme in nitrogen metabolism. Additionally, indications for a second glutamine synthetase, GSII, were found. |
| |
| Keywords: | Haloanaerobiaceae Haloanaerobium Taxonomy Oil field |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
