| 小球藻亲环蛋白A(CyPA)基因克隆及表达特性分析 |
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| 引用本文： | 王旭辉,乔坤,汪振娟,管清杰,柳参奎. 小球藻亲环蛋白A(CyPA)基因克隆及表达特性分析[J]. 植物研究, 2014, 34(2): 245-251 |
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| 作者姓名： | 王旭辉 乔坤 汪振娟 管清杰 柳参奎 |
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| 作者单位： | 东北油田盐碱植被恢复与重建教育部重点实验室，盐碱地生物资源环境研究中心，东北林业大学，哈尔滨 150040 |
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| 基金项目： | 中国博士后基金(2012M-520695);黑龙江省自然基金(留学及海外基金LC2013C10) |
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| 摘 要： | 亲环蛋白广泛存在于生物体中,并发挥着重要的作用。本研究从小球藻cDNA文库中筛选获得亲环蛋白A(CyPA Gene);通过提取转化35S::CyPA::GFP融合质粒拟南芥的原生质体研究表明,CyPA定位于叶绿体中;过表达CyPA基因的酵母能够增强InV酵母对NaCl、NaHCO3胁迫的生长能力;通过实时定量PCR研究表明,在非生物胁迫的条件下,CyPA基因的表达量升高,推测CyPA基因与非生物胁迫抗性相关,亲环蛋白A参与生物体的抗逆生存能力。
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| 关 键 词： | 小球藻 亲环蛋白 实时定量PCR 基因表达特性 |
Cloning and Expression Analysis of Cyclophilins A(CyPA) Gene form Chlorella |
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| Affiliation: | Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field(SAVER),Ministry of Education,Alkali Soil Natural Environmental Science Center(ASNESC),Northeast Forestry University,Harbin 150040 |
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| Abstract: | Cyclophilins (CyPs) are widely distributed in organisms, and play important roles. Cyclophilin A-like was screened in our study from Chlorella sp. cDNA library. By extracting Arabidopsis thaliana protoplasts that was transformed the 35S::CyPA::GFP fusion plasmid, it showed that CyPA located in the chloroplasts. Overexpression of CyPA gene in yeast enhanced the InV yeast growth ability to resistance of NaCl, NaHCO3 and drought stress. Real-time quantitative PCR showed that under abiotic stress conditions, CyPA gene expression quantity increased. So we speculated that CyPA genes were associated with abiotic stress resistance, through the cyclophilin A involved in improving survival ability of resistant organisms. |
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| Keywords: | chlorella cyclophilins real time quantitative PCR expression pattern of gene |
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