Application of protein-fragment complementation assays in cell biology |
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Authors: | Remy Ingrid Michnick Stephen W |
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Affiliation: | Département de Biochimie, Université de Montréal, Succursale Center-Ville, Montréal, QC, Canada. |
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Abstract: | We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology. |
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