Salmonella typhimurium InvA expression probed with a monoclonal antibody to the C-terminal peptide of InvA |
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Authors: | Clifford G Clark Leslie A MacDonald Christine C Ginocchio Jiorge E Galán Roger P Johnson |
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Institution: | Health of Animals Laboratory, Agriculture and Agri-Food Canada, 110 Stone Road West, Guelph, Ont. NIC 3W4, Canada; Department of Microbiology, School of Medicine, State University of New York at Stony Brook. Stony Brook, NY 11794, USA |
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Abstract: | Abstract The Salmonella typhimurium InvA protein is a component of a sec -independent secretion apparatus necessary for full virulence of the bacteria. We generated a monoclonal antibody to the C-terminal portion of the InvA protein that recognized proteins in S. typhimurium and weakly in Y. enterocolitica , but not in several other species of bacteria, including S. flexneri. S. typhimurium grown without agitation produced relatively constant amounts of membrane InvA throughout the growth cycle, whereas bacteria grown with agitation had a sharp increase in the amount of membrane InvA at late exponential phase. Levels of InvA present in Salmonella membranes under some growth conditions do not appear to correlate with levels of invasion under the same conditions. |
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Keywords: | Salmonella typhimurium InvA Invasion Monoclonal antibody |
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