Quantification of endogenous α- and γ-endorphins in rat brain by liquid chromatography-tandem mass spectrometry

Quantification of α- and γ-endorphins in rat brain using liquid chromatography-electrospray ionization-tandem mass spectrometry is described. [D-Ala²]-γ-endorphin is used as an internal standard. The precursor-to-product ion MRM transitions for α-endorphin, γ-endorphin, and [D-Ala²]-γ-endorphin were m/z 873.6 → 429.6; 929.6 → 542.3; 936.6 → 542.3, respectively. The method was validated in terms of linearity, specificity, sensitivity, recovery, precision, and accuracy. The assay was linear over a concentration range of 0.1-200 ng/mL with the limit-of-detection of 0.03 ng/mL and limit-of-quantification of 0.1 ng/mL. The endogenous concentrations of α- and γ-endorphins in rat brains were 13.8 ± 0.57 (mean ± SD; n = 5) and 2.5 ± 0.43 ng/g of wet tissue weight, respectively.