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A robust method of determination of high concentrations of peptides and proteins |
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| Authors: | Pavel A Levashov Duncan S Sutherland Stepan Shipovskov |
| Institution: | a Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark b Department of Chemical Enzymology, Chemical Faculty, Moscow State University, 119992 Moscow, Russia c Laboratory of Clinical Chemistry, Cardiology Research Center, 3rd Cherepkovskaya str. 15a, 121552 Moscow, Russia d Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark |
| Abstract: | In this paper, we pioneer application of a unique method of protein determination by coloring peptide bonds for analysis of a variety of biomolecules with different grades of purity (e.g., oligopeptides, membrane, and glycol proteins). We demonstrated that the calibration curve for all studied molecules is universal and linear within 0.1 to 1.2 mg protein content range. The assay thus can be used to analyze peptides without preliminary dilutions and calibration in up to 1 g/ml solutions of peptides, which is crucial for many biotechnological processes, such as development of coatings, scaffolds, and biocompatible materials. |
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