3-(10′-Phenothiazinyl)propane-1-sulfonate is a potent enhancer of soybean peroxidase-induced chemiluminescence |
| |
Authors: | Marina M. Vdovenko Ivan Yu. Sakharov |
| |
Affiliation: | a Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia b Cyanagen, 40138 Bologna, Italy c Division of Chemistry, G.V. Plekhanov Russian Economic Academy, 115998 Moscow, Russia |
| |
Abstract: | 3-(10′-Phenothiazinyl)propane-1-sulfonate (SPTZ) was shown to be a potent enhancer of soybean peroxidase (SbP)-induced chemiluminescence. To the best of our knowledge, SPTZ is the first enhancer of SbP to be discovered. Optimal conditions for SbP-catalyzed oxidation of luminol in the presence of SPTZ were determined. The SbP-SPTZ system showed better sensitivity and a lower detection limit (LDL) with respect to the horseradish peroxidase-4-iodophenol system traditionally used in chemiluminescent enzyme-linked immunosorbent assay (ELISA). The addition of 4-morpholinopyridine (MORP) to the SbP-SPTZ system improved its analytical parameters by decreasing the LDL of SbP to 0.03 pM. These results open up very promising perspectives for using the SbP-SPTZ-MORP system in ultrasensitive immunoassay. |
| |
Keywords: | Chemiluminescence Peroxidase Soya Enhancer 3-(10&prime -Phenothiazinyl)propane-1-sulfonate 4-Morpholinopyridine |
本文献已被 ScienceDirect 等数据库收录! |