A rapid procedure for the isolation of plasmid DNA from environmental bacteria. |
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| Authors: | M A Ferrús J L Alonso I Amorós M Hernández J Hernández |
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| Affiliation: | Department of Biotechnology, Polytechnic University of Valencia, Spain. mferrus@btc.upv.es |
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| Abstract: | The INSTA-MINI-PREP method, a rapid protocol for plasmid DNA extraction, was originally developed to prepare plasmid DNA from 1 to 3 ml miniprep Escherichia coli cultures. Direct extraction of plasmid DNA is achieved by a two-phase solution which is separated by centrifugation in the presence of the INSTA-PREP gel barrier material. This method has been successfully tested on various environmental Salmonella strains, although it was not suitable for Pseudomonas aeruginosa and enterococci strains. The INSTA-MINI-PREP method is a new alternative procedure to screen plasmid contents of Salmonella and E. coli strains rapidly and easily. |
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