Cellular basis of neurogenesis in the brain of crayfish,Procambarus clarkii: Neurogenic complex in the olfactory midbrain from hatchlings to adults |
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| Authors: | Cha-Kyong Song Laurel M. Johnstone Donald H. Edwards Charles D. Derby Manfred Schmidt |
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| Affiliation: | 1. Departament de Biologia Animal (Vertebrats), Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain;2. Departament de Salut Pública, Facultat de Medicina, Universitat de Barcelona, 08036 Barcelona, Spain;3. Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ, UK;4. Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona 08028, Spain;5. Department of Biology, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium;1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China;2. Wuxi Fisheries College, Nanjing Agricultural University, Nanjing 210095, PR China;1. Department of Life Sciences, University of Trieste, via L. Giorgieri, 5, 33127 Trieste, Italy;2. Fondazione Callerio, via Fleming 22-31/b, 34127 Trieste, Italy;3. International Centre for Genetic Engineering and Biotechnology, AREA Science Park, Trieste, Italy |
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| Abstract: | Neurogenesis in the central olfactory pathway of decapod crustaceans persists throughout life. Here we describe the structural basis of neurogenesis within the olfactory deutocerebrum of the crayfish Procambarus clarkii from hatchlings to adults. Using a proliferation marker and immunostaining, we found that throughout development each hemibrain contains a neurogenic complex consisting of five parts: two proliferation zones, each within the neuronal soma clusters containing local or projection interneurons, a tail of proliferating cells extending from each proliferation zone, and an elongated clump of cells where the two tails meet. The clump of cells comprises two subdivisions joined at a nucleus-free central area. Each subdivision consists of a dense group of clump cells with small, spindle-shaped nuclei and is connected to one of the proliferation zones by a strand of fibrous material encompassing the tail of proliferating cells extending from it. We identify one proliferating cell with a large nucleus in each subdivision as a putative neuroblast. Its daughter cells migrate through the strands to the associated proliferation zones, but in the strand leading to the soma cluster of local interneurons this is masked by local proliferation. We conclude that neurogenesis in the olfactory deutocerebrum of juvenile and adult P. clarkii is based on a few neuroblasts that are associated with unique clumps of cells likely representing stem cell niches. |
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