Human bone marrow mesenchymal cells express NG2: possible increase in discriminative ability of flow cytometry during mesenchymal stromal cell identification |
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| Authors: | Ilknur Kozanoglu Can Boga Hakan Ozdogu Oktay Sozer Erkan Maytalman Ayse Canan Yazici Feride Iffet Sahin |
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| Institution: | 1. Department of Physiology, Baskent University Faculty of Medicine, Ankara, Turkey;2. Baskent University Adana Teaching and Medical Research Center, Hematology Research Laboratory, Adana, Turkey;3. Department of Hematology, Baskent University Faculty of Medicine, Ankara, Turkey;4. Department of Biostatistics, Baskent University Faculty of Medicine, Ankara, Turkey;5. Department of Medical Genetics, Baskent University Faculty of Medicine, Ankara, Turkey;1. Department of Hematology, Tianjin Medical University Cancer Hospital and Institute, Tianjin, China;2. Department of Hematology, First Affiliated Hospital of General Hospital of the Chinese People''s Liberation Army, Beijing, China;1. Cell Engineering Laboratory, La Paz University Hospital Research Institute, Madrid, Spain;2. Department of Internal Medicine, La Paz University Hospital, Madrid, Spain;3. Department of Plastic and Reconstructive Surgery, Santa Cristina Hospital, and Centrocim, Madrid, Spain;4. Department of Pathology, La Paz University Hospital, Madrid, Spain;1. Department of Pathophysiology, Faculty of Medicine, University of Szeged, Hungary;2. Department of Endocrinology, Faculty of Medicine, University of Szeged, Hungary;3. Neuroscience Research Group of the Hungarian Academy of Sciences, Szeged, Hungary;1. Trinity College Institute of Neuroscience, Trinity College, Dublin 2, Ireland;2. Protein Laboratory, Institute of Neuroscience and Pharmacology, School of Medicine, University of Copenhagen, Copenhagen, Denmark;1. Institut Pasteur, Unité Biologie et Pathogénicité Fongiques – INRA USC2019, 75015 Paris, France;2. Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain;3. Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA;1. Children''s Research Institute and Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;2. Howard Hughes Medical Institute |
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| Abstract: | Background aimsMesenchymal stromal cells (MSC) exhibit non-specific hematopoietic cell and/or stromal cell markers (e.g. CD73, CD105 and CD166) that have been used to identify MSC by flow cytometry. Because a neural glial antigen, NG2 (a progenitor cell marker in the central nervous system), is expressed by several tissue cells originating in the mesenchyme but not hematopoietic cells, it might be useful for isolating and identifying MSC. We investigated NG2 expression on culture-expanded MSC by flow cytometry.MethodsHuman bone marrow (BM) samples taken from 12 donors were cultured for MSC to be used in up to nine serial passages. Using flow cytometry, the neural glial antigen NG2 and commonly used MSC markers CD73, CD105 and CD166, were analyzed on the surface of culture-expanded MSC. The multipotential differentiation of the MSC was examined by adipogenic and osteogenic induction.ResultsThe percentage of cells positive for NG2 was similar to the percentages of cells positive for CD73, CD105 and CD166 in all passages of BM samples. The mean fluorescent intensities of NG2 did not change with culture passage. The MSC was successfully differentiated into adipogenic and osteogenic lines. The cells showed no karyotypic abnormalities.ConclusionsNG2 seems to be a promising marker for investigating the biology of MSC. |
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