Comparison of freezing tolerance in cultured plant cells and their respective protoplasts |
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| Institution: | 1. Department of Horticulture, Colorado State University, Fort Collins, Colorado 80523 USA;2. Tissue Culture for Crops Project, Colorado State University, Fort Collins, Colorado 80523 USA;1. Cleveland Diagnostics, Cleveland, OH 44114, USA;2. Department of Molecular Medicine and USF Health Byrd Alzheimer''s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA;1. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, PR China;2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, PR China;1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan;2. Faculty of Agriculture, Niigata University, Niigata 950-2181, Japan;3. Food Component Analysis Unit, Food Research Institute, National Agriculture and Food Research Organization, 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8642, Japan;1. Centro de Estudos Florestais (CEF), Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal;2. Centro de Investigação em Agronomia, Alimentos, Ambiente e Paisagem (LEAF), Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal;3. Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal;4. Divisão de Clima e Alterações Climáticas, Departamento de Meteorologia e Geofísica, Instituto Português do Mar e da Atmosfera I. P. (IPMA, IP), Rua C do Aeroporto, 1749-077 Lisboa, Portugal |
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| Abstract: | The possibility that the plant cell wall influences the severity of freezing injury was examined by comparing the freeze stress response of intact cells and protoplasts from four different suspension cultures. In no case did the intact cells suffer more injury than the respective wall-less protoplasts, showing that mechanical strain imposed by the cell wall during freeze-thaw stress is not a major determinant of injury. For three of the four species studied, cells from which the wall was removed showed significantly greater freezing injury, indicating that the plant cell wall may have a protective role. Other researchers have suggested that cell wall rigidity may minimize freezing injury by slowing freeze-induced loss of cell water. We found that decreased enzyme digestibility (perhaps indicating greater rigidity) of cell walls accompanied cold acclimation in various tissues. These results provide impetus to research which will characterize low-temperature-induced cell wall modification in cold acclimating tissues. |
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