Nitrogen uptake kinetics of Prymnesium parvum (Haptophyte) |
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Affiliation: | 1. Equipe Environnement et Microbiologie, IPREM UMR CNRS 5254, Université de Pau et des Pays de l''Adour, IBEAS, BP 1155, 64013 Pau Cedex, France;2. Plateforme Bioinformatique Genotoul, UR875, Biométrie et Intelligence Artificielle, INRA, 31326 Castanet-Tolosan, France;3. UMR 9190 MARBEC IRD-Ifremer-CNRS, Université de Montpellier, Place Eugène Bataillon, Case 093, 34095 Montpellier Cedex 5, France;4. MIO, Institut Méditerranéen d''Océanologie, UMR 7294, F13288 Marseille, France;5. EPOC, UMR 5805, Université de Bordeaux, F33615 Pessac, France;6. GeT-PlaGe, Genotoul, INRA Auzeville, F31326 Castanet-Tolosan, France;7. Université de Pau et des Pays de l''Adour, Laboratoire de Mathématiques et de leurs Applications de Pau, UMR CNRS 5142, FED 4155 MIRA, Campus Montaury, 64600 Anglet, France |
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Abstract: | The uptake rates of different nitrogen (N) forms (NO3−, urea, and the amino acids glycine and glutamic acid) by N-deficient, laboratory-grown cells of the mixotrophic haptophyte, Prymnesium parvum, were measured and the preference by the cells for the different forms determined. Cellular N uptake rates (ρcell, fmol N cell−1 h−1) were measured using 15N-labeled N substrates. P. parvum showed high preference for the tested amino acids, in particular glutamic acid, over urea and NO3− under the culture nutrient conditions. However, extrapolating these rates to Baltic Seawater summer conditions, P. parvum would be expected to show higher uptake rates of NO3− and the amino acids relative to urea because of the difference in average concentrations of these substrates. A high uptake rate of glutamic acid at low substrate concentrations suggests that this substrate is likely used through extracellular enzymes. Nitrate, urea and glycine, on the other hand, showed a non-saturating uptake over the tested substrate concentration (1–40 μM-N for NO3− and urea, 0.5–10 μM-N for glycine), indicating slower membrane-transport rates for these substrates. |
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