In situ pulse respirometric methods for the estimation of kinetic and stoichiometric parameters in aerobic microbial communities |
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| Institution: | 1. IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal;2. Centro de Investigación y Estudios Avanzados del IPN (Cinvestav), Depto. Biotecnología, Av. IPN. 2508, San Pedro Zacatenco, C.P. 07360, Mexico DF, Mexico;1. Department of Automation and Energy, Universidade Federal do Rio Grande do Sul, Av. Osvaldo Aranha, 103, Porto Alegre, RS, Brazil;2. University of Mons – Automatic Control Laboratory – Biosys, Boulevard Dolez 31, 7000 Mons, Belgium;3. Equipe Projet INRA-INRIA MODEMIC, Route des Lucioles, 06902 Sophia-Antipolis, France;4. Service de Chimie et Biochimie Appliquées, Université de Mons, 56 Rue de l’épargne, B-7000 Mons, Belgium;5. UMR INRA-SupAgro MISTEA, 2 Place Viala, 34060 Montpellier, France;1. Chemical Engineering Faculty, Sahand University of Technology, Tabriz 51335-1996 Iran;2. Environmental Engineering Research Center (EERC), Sahand University of Technology, Tabriz 51335-1996 Iran;3. Department of Civil Engineering and Architecture, Technical University of Bari, Via Orabona, 4, 70125 Bari, Italy;1. Department of Automation, Electronics and Mechatronics, University of Craiova, A.I. Cuza 13, Craiova 200585, Romania;2. Department of Automatic Control, SUPÉLEC, Plateau de Moulon, 3 rue Joliot Curie, Gif sur Yvette 91192, France;3. Faculty of General Medicine, University of Medicine and Pharmacy “Carol Davila” Bucharest, Eroilor Sanitari 8, Bucharest 050474, Romania;1. Department of Chemistry, University of Patras, GR-26504 Patras, Greece;2. Department of Food Technology, TEI of Peloponnese, 24100 Kalamata, Greece;1. Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei, 066004, People''s Republic of China;2. CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026, People''s Republic of China |
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| Abstract: | In situ pulse respirometry was applied in an activated sludge bubble column treating synthetic wastewater for the estimation of the (i) maximum specific oxygen consumption rate, (ii) substrate affinity constant, (iii) biomass growth yield, (iv) maintenance coefficient, and (v) specific endogenous respiration rate. Parameters obtained from respirometry were compared to those obtained by the chemostat method, based on substrate and biomass measurements, under several dilution rates. The low sensitivity of substrate measurement methods and the difficulties of sampling heterogeneous biomass suspension are critical issues limiting the applicability of the chemostat method. Additionally, the extensive time consuming nature of this method allows concluding that chemostat method presents several disadvantages in comparison with in situ pulse respirometric techniques. Parameters were obtained from respirograms by fitting ASM1 and ASM3 models, and from experiments performed by injecting pulses of increasing substrate concentration. The injection of pulses of increasing concentration was the most adequate method, with several advantages such as a simpler experimental data interpretation, and results with better confidence.Considering the assessment and comparison of the experimental and calculation methods presented, it is recommended that the estimation of kinetic and stoichiometric parameters in mixed aerobic cultures should preferentially be performed by using in situ respirometric techniques. |
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