Transneuronal Dpr12/DIP‐δ interactions facilitate compartmentalized dopaminergic innervation of Drosophila mushroom body axons |
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Authors: | Bavat Bornstein Hagar Meltzer Ruth Adler Idan Alyagor Victoria Berkun Gideon Cummings Fabienne Reh Hadas KerenShaul Eyal David Thomas Riemensperger Oren Schuldiner |
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Institution: | 1. Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot Israel ; 2. Institute of Zoology, University of Cologne, Köln Germany ; 3. Department of Immunology, Weizmann Institute of Science, Rehovot Israel ; 4. Life Science Core Facility, Weizmann Institute of Science, Rehovot Israel |
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Abstract: | The mechanisms controlling wiring of neuronal networks are not completely understood. The stereotypic architecture of the Drosophila mushroom body (MB) offers a unique system to study circuit assembly. The adult medial MB γ‐lobe is comprised of a long bundle of axons that wire with specific modulatory and output neurons in a tiled manner, defining five distinct zones. We found that the immunoglobulin superfamily protein Dpr12 is cell‐autonomously required in γ‐neurons for their developmental regrowth into the distal γ4/5 zones, where both Dpr12 and its interacting protein, DIP‐δ, are enriched. DIP‐δ functions in a subset of dopaminergic neurons that wire with γ‐neurons within the γ4/5 zone. During metamorphosis, these dopaminergic projections arrive to the γ4/5 zone prior to γ‐axons, suggesting that γ‐axons extend through a prepatterned region. Thus, Dpr12/DIP‐δ transneuronal interaction is required for γ4/5 zone formation. Our study sheds light onto molecular and cellular mechanisms underlying circuit formation within subcellular resolution. |
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Keywords: | circuit formation dopaminergic neurons IgSF mushroom body compartments neuronal remodeling |
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