Immobilization of glucose oxidase enzyme (GOD) in large pore ordered mesoporous cage-like FDU-1 silica |
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| Institution: | 1. Departamento de Química Fundamental do Instituto de Química da Universidade de São Paulo, USP, C.P. 26077, CEP 05599-970, São Paulo, Brazil;2. Centro de Ciência e Tecnologia de Materiais do Instituto de Pesquisas Energéticas e Nucleares–IPEN, C.P. 11049, CEP 05422-970, São Paulo, Brazil;3. Departamento de Física Aplicada do Instituto de Física da Universidade de São Paulo, USP, CP 66318, CEP 05315-970, São Paulo, Brazil;1. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, Buenos Aires, Argentina;2. Universidad Nacional de Río Negro, Av. Don Bosco y Leloir (s/n), 8500 Viedma, Río Negro, Argentina;1. Francis Marion University, Department of Biology, P.O. Box 100547, Florence, SC 29502, USA;2. Michigan State University, Department of Entomology, 288 Farm Lane Room 243, East Lansing, MI 48824, USA;3. U.S. Geological Survey, Great Lakes Science Center, 1451 Green Road, Ann Arbor, MI 48105, USA |
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| Abstract: | Large pore ordered mesoporous silica FDU-1 with three-dimensional (3D) face-centered cubic, Fm3m arrangement of mesopores, was synthesized under strong acid media using B-50-6600 poly(ethylene oxide)–poly(butylene oxide)–poly(ethylene oxide) triblock copolymer (EO39BO47EO39), tetraethyl orthosilicate (TEOS) and trimethyl-benzene (TMB). Large pore FDU-1 silica was obtained by using the following gel composition 1TEOS:0.00735B50-6600:0.00735TMB:6HCl:155H2O. The pristine material exhibited a BET specific surface area of 684 m2 g?1, total pore volume of 0.89 cm3 g?1, external surface area of 49 m2 g?1 and microporous volume of 0.09 cm3 g?1. The enzyme activity was determined by the Flow Injection Analysis-Chemiluminescence (FIA-CL) method. For GOD immobilized on the FDU-1 silica, GOD supernatant and GOD solution, the FIA-CL results were 9.0, 18.6 and 34.0 U, respectively. The value obtained for the activity of the GOD solution with FIA-CL method is in agreement with the 35 U, obtained by spectrophotometry. |
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