Pathways and kinetics analysis of biotransformation of Dioscorea zingiberensis by Aspergillus oryzae |
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| Institution: | 1. State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, People’s Republic of China;2. Amoy-BUCT Industrial Bio-technovation Institute, Beijing University of Chemical Technology, Amoy, 361022, China;3. Kim Il Sung University, Pyongyang, 999095, North Korea;4. Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA;1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Long Pan Road, Nanjing 210037, China;2. College of Chemical Engineering, Nanjing Forestry University, 159 Long Pan Road, Nanjing 210037, China;3. Jiangsu Key Lab for the Chemistry & Utilization of Agricultural and Forest Biomass, 159 Long Pan Road, Nanjing 210037, China;4. Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, China |
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| Abstract: | In this paper, the pathways and kinetics for the production of diosgenin via biotransformation of Dioscorea zingiberensis C.H. Wright by Aspergillus oryzae CICC 2436 were analyzed. After 120 h of biotransformation at 30 °C, the concentration of diosgenin in the culture reached 36.87 ± 1.27 μmol/g raw herb, which was 21.2 times its initial concentration. A number of steroidal compounds were also isolated as minor products from the biotransformation, and one of these was identified as a novel compound named 3-O-β-d-glucopyranosyl (1 → 3) – β-d-glucopyranosyl (1 → 4) – β-d-glucopyranosyl-diosgenin (diosgenin-triglucoside). The biotransformation consisted of two stages: the release of steroids from the herb (accompanied by fungal growth) and hydrolysis of the steroids by glycosidases. Kinetic analysis and mathematical modelling showed that the process of biotransformation could be described by first-order kinetics under the condition of high Km/S] values. It consisted of a cascade of consecutive and parallel reactions involving three kinds of enzymes, five steroid saponins and their sapogenin. The main hydrolysis reactions that led to the production of diosgenin were also discussed. |
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