A simple plant polytene chromosome system,and its use for in situ hybridisation |
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| Authors: | David Roy Davies Christopher Ashley Cullis |
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| Institution: | (1) John Innes Institute, Colney Lane, NR4 7UH Norwich, England |
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| Abstract: | When the cotyledons of Pisum sativum are cultured in the presence of 2–4D, large populations of cells with polytene chromosomes are stimulated to enter prophase; the use of these chromosome for studies of in situ hybridisation with nucleic acids is described. |
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| Keywords: | Polytene chromosomes in situ hybrids Pisum |
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