Parameters of unbalanced growth and reversible inhibition of deoxyribonucleic acid synthesis in Brevibacterium ammoniagenes ATCC 6872 induced by depletion of Mn2+. Inhibitor studies on the reversibility of deoxyribonucleic acid synthesis |
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Authors: | Georg Auling Martin Thaler Hans Diekmann |
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Affiliation: | (1) Lehrgebiet für Mikrobiologie der Universität Hannover, Schneiderberg 50, D-3000 Hannover 1, Federal Republic of Germany;(2) Present address: Institut für Tierernährung der Universität Hohenheim, Postfach 106, D-7000 Stuttgart 70, Federal Republic of Germany |
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Abstract: | Unbalanced growth induced by depletion of manganese ions was a prerequisite for production of ribonucleotides in a high salt mineral medium with the wildtype strain Brevibacterium ammoniagenes ATCC 6872. The concentration of manganese strictly controlled the overall deoxyribonucleic acid (DNA) synthesis, whereas ribonucleic acid (RNA), protein and cell wall synthesis remained essentially unimpaired in the manganese-lacking cells.The reversibility of inhibition of overall DNA synthesis was shown by enhanced incorporation (up to threefold compared to the cultures supplied with sufficient manganese) of [8-14C] adenine into alkali-stable, trichloroacetic acid-insoluble material after subsequent addition of 10 M MnCl2 to 15 h-old depleted cultures.The results of inhibitor studies on the restoration of overall DNA synthesis due to subsequent addition of manganese ions to depleted cultures suggest that ribonucleotide reduction is the primary target of the manganese starvation during nucleotide fermentation with Brevibacterium ammoniagenes ATCC 6872. |
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Keywords: | Manganese deficiency Unbalanced growth Brevibacterium ammoniagenes Reversible inhibition of DNA synthesis Inhibition by hydroxyurea Mn2+-Controlled ribonucleotide reduction |
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