Purification of alpha 2,6-sialyltransferase from rat liver by dye chromatography. |
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| Authors: | U Sticher H J Gross R Brossmer |
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| Affiliation: | Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middx, U.K. |
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| Abstract: | We describe a simple three-step purification for Gal-beta 1,4-GlcNAc-alpha 2,6-sialyltransferase (EC 2.4.99.1) from rat liver which uses chromatography on Cibacron Blue F3GA and f.p.l.c. It gives a highly purified (11,000-fold) enzyme in 19% yield, which is free of other sialyltransferases, CMP-NeuAc hydrolase, sialidases and proteinases. |
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