Glycoconjugates and keratin 18 define subsets of taste cells |
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Authors: | Q Zeng A Lawton and B Oakley |
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Institution: | (1) Department of Biology, University of Michigan, 48109 Ann Arbor, MI, USA |
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Abstract: | Summary Sections of neonatal, normal adult and denervated adult rat tongue were examined with lectin histochemistry. Attention was
focused upon intragemmal cells (cells within the taste bud) and the surrounding perigemmal cells. Informative staining patterns
were observed with four of 12 lectins:Ulex europaeus (UEA-I),Bauhinia purpurea (BPA),Helix pomatia (HPA) andLotus tetragonolobus (LTA) agglutinins. In normal adult tongues, BPA bound to those lingual epithelial cells lacking contact with the basal lamina.
After they formed, vallate taste buds were laterally surrounded by distinctive BPA-positive cells. HPA reacted selectively
with 28% and LTA with 23% of the intragemmal cells in vallate/foliate taste buds. In double-stained taste buds there was,
a statistically significant overlap of LTA-positive cells and keratin 18-positive cells. The overlap between HPA binding and
keratin 18 was more marked: double-stained cells comprized 67% of all stained cells. During taste bud development in neonates
keratin 18 synthesis preceded HPA binding. In contrast, during the replacement of adult taste cells, keratin 18 synthesis
and HPA binding were generally concurrent. Keratin 18 and HPA probably identify the same subset of older taste receptor cells.
HPA may bind to glycoconjugates on the surface of keratin 18-positive cells. In denervated adult tongue the loss of all UEA-I-positive
or BPA-positive perigemmal cells suggests that perigemmal as well as intragemmal cells are nerve-dependent. |
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