Characterization of a novel <Emphasis Type="Italic">cry8</Emphasis> gene specific to Melolonthidae pests: <Emphasis Type="Italic">Holotrichia oblita</Emphasis> and <Emphasis Type="Italic">Holotrichia parallela</Emphasis> |
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| Authors: | Changlong Shu Guixin Yan Rongyan Wang Jie Zhang Shuliang Feng Dafang Huang Fuping Song |
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| Institution: | (1) State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China;(2) Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding, 071000, People’s Republic of China;(3) Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China; |
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| Abstract: | A new polymerase chain reaction–restriction fragment length polymorphism method for the identification of cry8-type genes from Bacillus thuringiensis has been established by designing a pair of new universal primers. By this method, a novel gene, cry8Ga1, encoding a polypeptide of 1,157 amino acids with a deduced molecular mass of 131.2 kDa was identified and cloned from B. thuringiensis HBF-18. Recombinant B. thuringiensis strain HD8G, harboring cry8Ga1, has insecticidal activity against larvae of Melolonthidae pests: Holotrichia oblita and Holotrichia parallela. This is the first report of a Cry toxin that has insecticidal activity to Melolonthidae pest H. oblita. |
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