Zinc accumulation patterns in four Anthyllis vulneraria subspecies supplemented with mineral nitrogen or grown in the presence of their symbiotic bacteria |
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Authors: | Souhir Soussou Stéphanie Mahieu Brigitte Brunel José Escarré Michel Lebrun Mohamed Banni Hamadi Boussetta Jean-Claude Cleyet-Marel |
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Institution: | 1. INRA, Montpellier SupAgro, IRD, CIRAD, UM2, UMR113, Laboratoire des Symbioses Tropicales et Méditerranéennes, TA A-82/J, 34398, Montpellier cedex 5, France 3. Montpellier SupAgro, UMR113, 34398, Montpellier cedex 5, France 5. Laboratoire de Biochimie et de Toxicologie environnementale, Institut Supérieur Agronomique de Chott-Mariem, 4042, Chott-Mariem, Sousse, Tunisia 2. CNRS, Centre d’Ecologie Fonctionnelle et Evolutive, UMR 5175, 34293, Montpellier Cedex 05, France 4. UM2, UMR113, 34398, Montpellier cedex 5, France
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Abstract: | Aims This work examines Zn accumulation in four Anthyllis vulneraria subspecies supplemented with mineral nitrogen or grown in the presence of their symbiotic bacteria. Methods Anthyllis vulneraria subspecies were grown hydroponically in the presence of high levels of ZnSO4. The plants were either grown in symbiosis with one of two non-metallicolous or metallicolous Mesorhizobium inoculants or in the presence of KNO3. Results When exposed to 1,000 μM Zn, shoot and root biomass of three out of our four Anthyllis subspecies cultivated with NO3 dropped significantly by about 24–28 %; carpatica, the fourth subspecies, was not affected. Subspecies carpatica Zn tolerance was confirmed when in symbiosis with the metallicolous strain. In the presence of 1,000 μM Zn, the different Anthyllis subspecies concentrated more Zn in their roots than in their shoots and only subsp. carpatica accumulated a significant amount of Zn in its shoots. The most remarkable feature was the drastic decrease in Zn concentration in both roots (up to 2.5–3 fold) and shoots (2.6-fold) of subsp. carpatica exposed to 1,000 μM Zn and nodulated whatever the Mesorhizobium strain used, compared to the N-grown plants. Conclusions Our results bring new perspectives as regards phytostabilization, with the potential use of a rhizobium-inoculated leguminous subspecies displaying unusual Zn tolerance. |
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