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Ethylene as an endogenous inhibitor of root regeneration in tomato leaf discs cultured in vitro
Authors:WARREN K COLEMAN  TERRY J HUXTER  DAVID M REID  TREVOR A THORPE
Institution:Department of Biology, The University of Calgary, Calgary, Alberta, Canada T2N 1N4
Abstract:We examined ethylene effects on root regeneration in tomato leaf discs cultured in vitro. Applied ethylene or Ethephon did not stimulate rooting in the leaf discs. In the presence of indoleacetic acid. 5 × 10-6M, these substances significantly inhibited root formation. Ethylene production (nl C2H4· (24 h)-1. flask-1) was positively correlated with increased IAA concentrations at various times during the culture period and, as a consequence, with the rooting response after 168 h. However, separate testing of equimolar concentrations of seven different auxins and auxin-like compounds showed no positive correlation between the rate of ethylene production and subsequent rooting response. Aeration of gas-tight flasks containing leaf discs and absorption of ethylene evolved from the discs by mercuric perchlorate in gas-tight flasks or pre-treatment of leaf discs with AgNO3 significantly enhanced IAA induced root regeneration. Thus, these studies indicate that ethylene is not a rooting hormone per se. Furthermore, ethylene (whether applied externally or synthesized by the tissue) does not appear to account for the ability of auxin to stimulate rooting.
Keywords:Ethylene  rooting  tomato  leaf discs culture
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