Stimulation of spreading of trypsinized human fibroblasts by lysozymes fromStaphylococcus aureus,hen egg white,and human urine |
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Authors: | G Satta B Azzarone P E Varaldo R Fontana S Valisena |
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Institution: | (1) Istituto di Microbiologia dell’Università di Genova, Viale Benedetto XV, 10, 16132 Genova, Italy |
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Abstract: | Summary The effect of lysozyme from three different sources—Staphylococcus aureus, hen egg white, and human urine—on adhesion to substrate and spreading of trypsinized human fibroblasts was studied. Several
fibroblast strains were tested under various conditions. It was found that the different cell strains did not show the same
capability of spreading and stably attaching to substrates when resuspended in media not containing serum. Some strains did
not spread, whereas others spread even in the absence of serum. Cell spreading in these strains did not occur when the cells
were pregrown for 5 weeks in media supplemented with 1% fetal bovine serum. Lysozyme fromS. aureus allowed stable adhesion to substrate and spreading of all the fibroblast strains unable to elongate in nonsupplemented minimal
essential medium. This enzyme accelerated and augmented spreading of the strains capable of elongating in the absence of serum.S. aureus lysozyme also allowed spreading and stable adhesion to substrates of all these strains when they were pregrown for 5 weeks
in the presence of 1% fetal bovine serum. Furthermore, hen egg white lysozyme and the lysozyme purified from human urine were
both capable of stimulating anchorage to substrate and spreading of trypsinized fibroblasts although their effect was less
pronounced than that of theS. aureus lysozyme. Some tentative hypotheses for the mechanism of cell spreading in the presence of lysozyme are made. The possibility
that lysozymes, virtually ubiquitous enzymes, may play a specific role in nature in the regulation of cell differentiation
and tissue development is finally raised and discussed in light of several previous observations and findings.
This work was supported by Grant 79.00677.96 of Piano Finalizzato Tumori by the Consiglio Nazionale delle Ricerche of Italy. |
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Keywords: | lysozyme cell anchorage and spreading cell differentiation |
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