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Fusion of neurohypophyseal membranes in vitro
Authors:Manfred Gratzil  Gerhard Dahl  James T. Russel  Niels A. Thorn
Affiliation:1. Departments of Physiological Chemistry and Physiology I, University of Saarland, 6650 Homburg/Saar, G.F.R.;2. Institute of Medical Physiology C, University of Copenhagen, Copenhagen, Denmark
Abstract:Freeze cleaving electron microscopy has shown that fusion of isolated secretory vesicles from bovine neurohypophyses was induced by Ca2+ in micromolar concentrations. Mg2+ and Sr2+ were ineffective. Mg2+ inhibited Ca2+-induced fusion.In suspensions containing secretory vesicles as well as sheets of cell membrane, release of vasopressin parallel to intervesicular fusion of secretory vesicles with sheets of cell membrane was observed after exposure to Ca2+. Mg2+ and Sr2+ were ineffective in replacing Ca2+ as trigger for fusion or vasopressin release.Intervesicular fusion and exocytotic profiles were observed when isolated neurohypophyses or neurosecretosome were exposed to cold.
Keywords:Address correspondence to: Prof. Dr. Manfred Gratzl   Department of Physiological Chemistry   University of Saarland   D-6650 Homburg/Saar   G.F.R.
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