The mechanism of energy conservation and transduction by mitochondrial cytochrome c oxidase |
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Authors: | Mårten K.F. Wikström Herkko T. Saari |
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Affiliation: | Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10A, SF-00170 Helsinki 17 Finland |
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Abstract: | Oxidation of ferrocytochrome c by molecular oxygen catalysed by cytochrome c oxidase (cytochrome aa3) is coupled to translocation of H+ ions across the mitochondrial membrane. The proton pump is an intrinsic property of the cytochrome c oxidase complex as revealed by studies with phospholipid vesicles inlayed with the purified enzyme. As the conformation of cytochrome aa3 is specifically sensitive to the electrochemical proton gradient across the mitochondrial membrane, it is likely that redox energy is primarily conserved as a conformational “strain” in the cytochrome aa3 complex, followed by relaxation linked to proton translocation. Similar principles of energy conservation and transduction may apply on other respiratory chain complexes and on mitochondrial ATP synthase. |
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Keywords: | FCCP HEPES NEM TMPD Tricine WB Wurster's blue 1799 α,α′-bis(hexafluoracetonyl)acetone |
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