Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75005 Paris France
Abstract:
P-700, plastocyanin and cytochrome f redox kinetics were measured after one flash, using dark-adapted Chlorella in the presence of hydroxylamine and 3(3,4-dichlorophenyl)-1,1-dimethylurea. Plastocyanin becomes increasingly oxidized with a half-time of 70 μs, then undergoes reduction with a half-time of 7 ms. Cytochrome f oxidation has a sigmoidal time-course and a half-time of 100 μs. Its reduction exhibits a half-time of 4 ms. These results are interpreted in a linear scheme:
