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Uncoupler-induced changes in mitochondrial structure detected by small-angle X-ray scattering
Authors:Carmen A. Mannella  Donald F. Parsons
Affiliation:Electron Optics Laboratory, Roswell Park Memorial Institute, Buffalo, N.Y. 14263 U.S.A.
Abstract:Small-angle X-ray scattering data suggest that major but reversible rearrangements of mitochondrial inner membrane structure are induced by uncouplers. Low levels of 2,4-dinitrophenol (10 μM) cause a perceptible wide-angle shift of the 20 mrad X-ray scattering maximum characteristic of intact liver mitochondria. Higher dinitrophenol concentrations (> 25 μM) reduce this scattering maximum to one-third its initial intensity. In terms of mitochondrial function, the former scattering change appears to correlate with the uncoupling of oxidative phosphorylation while the latter occurs in the course of dinitrophenol stimulation of mitochondrial ATPase activity.
Keywords:DNP  2,4-dinitrophenol  CCCP
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