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A one microsecond component of chlorophyll luminescence suggesting a primary acceptor of system II of photosynthesis different from Q
Authors:JA Van Best  LNM Duysens
Institution:Department of Biophysics, Huygens Laboratory of the State University, Wassenaarseweg 78, Leiden The Netherlands
Abstract:The kinetics of the luminescence of chlorophyll a in Chlorella vulgaris were studied in the time range from 0.2 μs to 20 μs after a short saturating flash (t12 = 25 ns) under various pretreatment including anaerobiosis, flashes, continuous illumination and various additions. A 1 μs luminescence component probably originating from System II was found of which the relative amplitude was maximum under anaerobic conditions for reaction centers in the state SPQ? before the flash, about one third for centers in the state S+PQ? or SPQ before the flash, and about one tenth for centers in the state S+PQ before the flash. S is the secondary donor complex with zero charge; S+ is the secondary donor complex with 1 to 3 positive charges; P, the primary donor, is the photoactive chlorophyll a, P-680, of reaction center 2; Q? is the reduced acceptor of System II, Q. Under aerobic conditions, where an endogenous quencher presumably was active, the luminescence was reduced by a factor two.The 1 μs decay of the luminescence is probably caused by the disappearance of P+ formed in the laser flash according to the reaction ZP+ → Z+P in which Z is the molecule which donates an electron to P+ and which is part of S. After addition of hydroxylamine, the 1 μs luminescence component changed with the incubation time exponentially (τ = 27 s) into a 30 μs component; during the same time, the variable fluorescence yield, measured 9 μs after the laser flash, decreased by a factor 2 with the same time constant. Hereafter in a second much slower phase the fluorescence yield decreased as an exponential function of the incubation time to about the dark value; meanwhile the 30 μs luminescence increased about 50% with the same time constant (τ = 7 min). Heat treatment abolished both luminescence components.The 1 μs luminescence component saturated at about the same energy as the System II fluorescence yield 60 μs after the laser flash and as the slower luminescence components. From the observation that the amplitude is maximum if the laser flash is given when the fluorescence yield is high after prolonged anaerobic conditions (state SQ?), we conclude that the 1 μs luminescence is probably caused by the reaction
PWQ?+hv → P1WQ?P+W?Q?P1WQ? → PWQ?+hv
in which W is an acceptor different from Q. The presence of S+ reduced the luminescence amplitude to about one third. Two models are discussed, one with W as an intermediate between P and Q and another, which gives the best interpretation, with W on a side path.
Keywords:DCMU  3-(3′4′-dichlorophenyl)-1  1-dimethylurea
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