Subunits of the calcium ion-pump system of sarcoplasmic reticulum |
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Authors: | Vitor MC Madeira |
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Institution: | Departamento de Zoologia, Universidade de Coimbra, Coimbra Portugal |
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Abstract: | Sarcoplasmic reticulum membranes with high content of Ca2+-ATPase (80% of total protein) were dissolved in a non ionic medium and were submitted to isoelectric focusing in polyacrylamide gels. The membrane protein was resolved into six main bands whose isoelectric points range from 6 to 5. The mol. wt. of these peptides is about 100 000 as estimated by second dimension electrophoresis in sodium dodecyl sulfate-polyacrylamide system. The electrophoretic behaviour of the purified ATPase enzyme is similar to that of crude membranes. |
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Keywords: | TEMED N N′ N′-tetramethyl-1 2-diaminoethane |
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