Changes in structural organization of surface membrane during erythrocyte maturation |
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Authors: | Masayasu Inoue Kenji Okajima Kazunobu Ito Kozo Utsumi Satimaru Seno |
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Affiliation: | 1. Department of Biochemistry, Kumamoto University Medical School, Kumamoto, Japan;2. Department of Biochemistry, Cancer Institute, Okayama University Medical School, Okayama, Japan;3. Department of Pathology, Okayama University Medical School, Okayama Japan |
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Abstract: | The effect of concanavalin A and its succinylated derivative on cell agglutination and potassium compartmentation of mature and immature erythrocytes was observed. The binding of tetravalent concanavalin A to the surface glycoproteins of rabbit erythrocytes leads to a change in the properties of the surface membrane, which results in an induction of cell agglutination and concomitant release of potassium from the cells. Both of the phenomena induced by concanavalin A are temperature dependent, and observed at above 15°C.Divalent succinylated concanavalin A, lacking the inducing activity of surface glycoprotein cross-linking into patches and caps, caused neither cell agglutination nor change in the potassium compartmentation of erythrocytes and reticulocytes.In the case of immature reticulocytes, however, remarkable agglutination of the cells was induced without a change in the potassium compartmentation after treatment with tetravalent concanavalin A.It is suggested that changes in the molecular organization of the surface membrane occur in which potassium compartmentation of the reticulocytes becomes more susceptible to surface glycoprotein cross-linking during cellular maturation. |
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