Two plant-type ferredoxins from a blue-green alga, Nostoc verrucosum |
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Authors: | Masateru Shin Michiko Sukenobu Reiko Oshino Yoshiji Kitazume |
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Affiliation: | 1. Laboratory of Biochemistry, Kobe Yamate Women''s College, Kobe University, Kobe Japan;2. Department of Biology, Faculty of Science, Kobe University, Kobe Japan |
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Abstract: | Two plant-type ferredoxins were isolated and purified from a blue-green alga, Nostoc verrucosum. They were separable by chromatography on a DEAE-cellulose column. The slow-moving band was designated ferredoxin I (Fd I) and the fast-moving band was ferredoxin II (Fd II). The ratio of the yield of ferredoxins I and II was about 1:0.84. Both ferredoxins had absorption spectra similar to those of plant-type ferredoxins. Two atoms of non-heme iron and two of labile sulfur were found per mol of both ferredoxin I and ferredoxin II. Their molecular weights were identical and estimated to be about 18 000 by a gel filtration method. The biochemical activities of these Nostoc ferredoxins were studied: the NADP photoreduction activity on one hand and the NADP-cytochrome c reductase activity on the other. |
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