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Analysis of single nucleotide polymorphisms in three chromosomes of European sea bass Dicentrarchus labrax
Authors:Heiner Kuhl  Mbaye Tine  Jochen Hecht  Florian Knaust  Richard Reinhardt  
Institution:a Max Planck Institute for Molecular Genetics, Ihnestr. 63, D-14195 Berlin, Germany;b BCRT, Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany
Abstract:Single nucleotide polymorphisms (SNPs) are believed to contain relevant information and have been therefore extensively used as genetic markers in population and conservation genetics, and molecular ecology studies. This study reports on the identification of potential SNPs in a diploid European sea bass Dicentrarchus labrax genome by using reference sequences from three assembled chromosomes and mapping all WGS datasets onto them (3× Sanger, 3× 454 and 20× SOLEXA). A total of 20,779 SNPs were identified over the 1469 gene loci and intergenic space analysed. Within chromosomes the occurrence of SNPs was the lowest in exons and higher in introns and intergenic regions, which may be explained by the fact, that coding regions are under strong selective pressure to maintain their biological function. The ratio of nonsynonymous to synonymous mutations was smaller than one for all the chromosomes, suggesting that most of deleterious nonsynonymous mutations were eliminated by negative selection. SNPs were not uniformly distributed over the chromosomes. Two chromosomes exhibited large regions with extremely low SNP density, which might represent homozygous regions in the diploid genome. The results of this study show how SNP detection can take profit from sequencing a single diploid individual, but also uncover the limits of such an approach. SNPs that have been identified will support marker development for genetic linkage mapping, population genetics and aquaculture related questions in general.
Keywords:Genomics  Second generation sequencing  SNP  Teleost
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