Factors influencing somatic embryo maturation, high frequency germination and plantlet formation in Terminalia chebula Retz. |
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Authors: | C Anjaneyulu C C Giri |
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Institution: | (1) Centre for Plant Molecular Biology (CPMB), Department of Genetics and Biotechnology, Osmania University, Hyderabad, Andhra Pradesh, 500007, India |
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Abstract: | The factors influencing somatic embryo maturation, high frequency somatic embryo germination, and plantlet formation were
studied in Terminalia chebula Retz. Maturation of somatic embryo were influenced by a number of factors such as in vitro culture passage, concentrations
of sucrose, levels of abscisic acid (ABA), basal media and media additive combinations. Maximum frequency of somatic embryo
maturation (57.22 ± 2.02), was obtained on MS medium supplemented with 50 g/l sucrose. Different factors such as strengths
of MS nutrients, plant growth regulators, media additives and their combinations controlling somatic embryo germination and
plantlet formation were studied. High frequency of germination and plantlet formation (58.80 ± 1.47) were achieved by subsequent
subculture of mature somatic embryos on MS medium containing 30 g/l sucrose and 0.5 mg/l benzyladenine (BA). However, although
duration of in vitro passage of the callus tissue was critical, contribution of the combinations of plant growth regulators
and media additives showed nugatory effect on somatic embryo maturation and germination as evident from variable responses. |
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Keywords: | Somatic embryogenesis Plant growth regulators Media additives Maturation Germination Plantlet formation |
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