Denitrification in Rhodopseudomonas sphaeroides f. denitrificans |
| |
| Authors: | Balaram Kundu D J D Nicholas |
| |
| Institution: | (1) Department of Agricultural Biochemistry, Waite Agricultural Research Institute, University of Adelaide, 5064 Glen Osmond, South Australia, Australia |
| |
| Abstract: | Rhodopseudomonas sphaeroides f. denitrificans grown photosynthetically with NO
3
-
under anaerobic conditions accumulated NO
2
-
in the culture medium. In washed cells succinate, lactate, fumarate, citrate and malate, were effective electron donors for the reduction of NO
3
-
, NO
2
-
and N2O to N2 gas. Nitrate reductase was inhibited by amytal and potassium thocyanate. Nitrite reductase activity was severely restricted by potassium cyanide, sodium diethyldithiocarbamate, Amytal and 2-n-heptyl-4-hydroxyquinoline-N-oxide whereas N2O reductase was inhibited by NaN3, C2H2 and KCNS. Cells incubated with either K15NO3 or K15NO2 produced 15N2O and 15N2. A stoichiometry of 2:1 was recorded for the reduction of either NO
3
-
or NO
2
-
to N2O and N2 and for N2O to N2 it was 1:1.Abbreviations BVH
reduced benzyl viologen
- MVH
reduced methyl viologen
- HOQNO
2-n-heptyl-4-hydroxyquinoline-N-oxide
- CCCP
carbonyl cyanide-m-chlorophenyl-hydrazone
- DIECA
diethyl dithiocarbamate
- KCN
potassium cyanide |
| |
| Keywords: | Rhodopseudomonas sphaeroides f denitrificans Photodenitrifier Denitrification Nitrate reductase Nitrite reductase Nitrous oxide reductase |
| 本文献已被 SpringerLink 等数据库收录！ |
|
