小鼠胚胎发育过程中表皮生长因子受体的免疫荧光定位研究

用间接免疫荧光法研究了第12—19天小鼠胚胎的表皮生长因子受体(EGFR)在各种组织中的分布。结果表明,特异性荧光出现在EGFR阳性细胞的细胞膜上。第14.5天鼠胚鼻粘膜上皮首先显示很强的EGFR特异性荧光,此后荧光稍为减弱,直至第19天后消失。消化系统中,舌味觉上皮在第16天、胃粘膜上皮在第17—18天间、十二指肠上皮在第17.5—18.5天、直肠粘膜上皮在第15.5—16天和肛管粘膜上皮在第15天均显示强特异性荧光。肝细胞从第14.5天起有弱阳性反应,随胎龄增大强度缓慢地增强。此外,在胚胎发育不同时期,还看到若干组织呈现阳性反应,包括膀胱粘膜变移上皮、眼睑原基、腺垂体上皮、舌下腺腺泡细胞、附属腺上皮细胞、胰岛细胞、颌下腺腺上皮和导管上皮细胞、降主动脉内皮以及卵巢髓质部富含血管的疏松结缔组织中的成纤维细胞等。

IMMUNOFLUORESCENT LOCALIZATION OF EPIDERMALGROWTH FACTOR RECEPTOR DURINGDEVELOPMENT OF MOUSE FETUS

Using indirect immunofluorescent method, we have studied the localization of epidermal growth factor receptor (EGFR) in mouse fetus. Specific fluorescent was found on the cell membrane of various tissues at the different development stages of mouse fetus, such as the nasal mucous membrane epithelial cells in the 14.5th to 19th days, the glossal epithelial cells on day 16, the vascular epithelial cells of aorta descendens on day 18.5,the stomachal mucous membrane epithelium between the 17th and 18th days, the duodenal epithelial cells between days 17.5 and 18, the rectal mucous membrane epithelial cells and the vesical transitional epithelium between the 15.5th and 16th days and in the hepatic cells in the 14.5th to 19th days. EGFR positive cells were also observed in the anal tube, primodium of eye lid, adenohypophysial glandular epithelium, sublingual gland, adrenal medulla, ovarial medulla, submaxillary gland, glandula accessoria and pancreas islet cells.