An improved sample preparation method for analyzing mycobacterial proteins in two-dimensional gels |
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Authors: | D Bisht N Singhal P Sharma K Venkatesan |
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Institution: | (1) Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research), Tajganj, Agra, 282 001, India |
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Abstract: | Two-dimensional gel electrophoresis (2-DE) is currently a widely used analytical method for resolving complex mixtures of proteins. Sample preparation has a marked influence on 2-DE pattern. To reduce impurities and to increase the low-abundance proteins, protein precipitation is often used for the preparation of samples before 2-DE. In this study, we revealed that addition of SDS prior to TCA precipitation of mycobacterial cell extract proteins increases the resolution of the 2-D gel pattern. |
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Keywords: | mycobacteria tuberculosis protein precipitation two-dimensional gel electrophoresis |
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