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RAPD and ISSR markers indicate diminished gene flow due to recent fragmentation of Polylepis australis woodlands in central Argentina |
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| Authors: | Norma Julio Ana Sobral Juan Rondan Dueas Julio Di Rienzo Daniel Renison Isabell Hensen |
| Institution: | aDepartment of Population Genetics and Evolution, National University of Córdoba, Velez Sarsfield 299, 5000 Córdoba, Argentina bDepartment of Statistics, Agricultural College, National University of Córdoba, Av. Valparaíso s/n at University Campus, cc509, 5000 Córdoba, Argentina cDepartment of Ecology, University of Córdoba, Velez Sarsfield 299, 5000 Córdoba, Argentina dMartin-Luther-University Halle-Wittenberg, Institute of Geobotany and Botanical Garden, Am Kirchtor 1, D-06108 Halle/Saale, Germany |
| Abstract: | Two Polylepis australis BITT. populations differing in size were examined with the aim of determining future sampling strategies; assessing levels of genetic diversity and checking whether trees of different ages might vary in their genetic structure due to the effects of fragmentation. RADP and ISSR gave similar values of diversity. A re-sampling technique showed that for P. australis, 10 trees and 20 markers were enough to produce an unbiased estimator of heterozygosity. AMOVA suggested differences in allele frequencies between young and old trees in the small population (p = 0.052), but not in a large population (p = 0.864); suggesting that gene flow between the areas diminished in relatively recent times. This assumption is supported by the fact that allele frequencies among both woodlands were significantly different between the young (p < 0.0001), but not the older trees (p = 0.87). |
| Keywords: | Genetic diversity Sample size Comparison of techniques Connectivity |
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