| 糖基化、非糖基化、重组PAI－1功能和性质的比较 |
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| 引用本文： | 孙幼华,张欣平,丁皓,查锡良,宋后燕,朱运松.糖基化、非糖基化、重组PAI－1功能和性质的比较[J].生物化学与生物物理学报,1996(2). |
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| 作者姓名： | 孙幼华 张欣平 丁皓 查锡良 宋后燕 朱运松 |
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| 作者单位： | 上海医科大学基础医学院分子遗传学研究室 |
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| 摘 要： | 对从ＨｅｐＧ２细胞培养液中分离得到植基化和非糖基化ＰＡＩ－１（１型纤溶酶原激活物抑制剂），以及从ｐＹＺＨＢＩ－６６表达菌中纯化的非糖基化重组ＰＡＩ－１的某些性质和功能进行比较，结果显示，糖基化ＰＡＩ－１对ｔＰＡ（组织型纤溶酶原激活物）有较强的抑制，能较显著地被蛋白质变性剂所激活，对热有较强的稳定性，糖基化与非糖基化ＰＡＩ－１在ｐＨ２．５－９．０的范围内都相当稳定。纤维蛋白原和肝素能明显提高两者对ｔＰＡ的抑制作用。
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| 关 键 词： | 糖基化PAI－1 非糖基化PAI－1 重组PAI－1 寡糖 |
Comparative Studies on the Functions and Characteristics of the Glycosylated,the Nonglycosylated and the Recombinant PAI-1 |
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| SUN You-Hua, ZHANG Xin-Ping, DING Hao, ZHA Xi-Liang, SONG Hou-Yan and ZHU Yun-Song.Comparative Studies on the Functions and Characteristics of the Glycosylated,the Nonglycosylated and the Recombinant PAI-1[J].Acta Biochimica et Biophysica Sinica,1996(2). |
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| Authors: | SUN You-Hua ZHANG Xin-Ping DING Hao ZHA Xi-Liang SONG Hou-Yan and ZHU Yun-Song |
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| Abstract: | Glycosylated and nonglycosylated forms of PAI-1 were separated from HepG 2 cell by immunoaffinity chromatography with anti-PAI-1 monoclonal antibody,and the recombinant PAI-1 were purified from the media of expressed bacteria line of pYZhBI-66.Some functions and characteristics of the three PAI-1 were compared, such as the inhibiting effect on tPA, the activation by denaturation,the stability to thermal or pH changes, and the activation by fibrinogen and heparin. The results emphasized the roles of the oligosaccharide. |
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| Keywords: | Glycosylated PAI-1 Nonglycosylated PAI-1 rPAI-1 Oligosaccharide |
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