Herpetomonas samuelpessoai: electron microscopy and cytochemistry of electron-dense granules. |
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Authors: | T U de Carvalho T Souto-Padrón W de Souza |
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Affiliation: | Laboratórios de Ultra-estrutura Celular e Microscopia Eletrônica, Instituto de Biofísica, Universidade Federal do Rio de Janeiro, Cidade Universitária, 21.910, Rio de Janeiro, RJ, Brasil |
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Abstract: | Herpetomonas samuelpessoai has membrane-bound electrondense granules in its cytoplasm. The electron density independs on postfixation with osmium tetroxide and is enhanced by uranyl acetate staining. The granules contain iron, have basic proteins cytochemically detected by the silver ammoniacal method, and have a peroxidase activity as detected by the diaminobenzidine method. Some of the granules also have acid phosphatase activity. It is suggested that the granules may represent either lysosomes or a storage form of tetrapyrrole derivatives which are essential for the growth and metabolism of most Trypanosomatidae. |
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Keywords: | Protozoa parasitic Trypanosomatidae Electron-dense granules Electron microscopy Cytochemistry |
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