A technique for distinguishing between bacterial and non-bacterial respiration in decomposing Spartina alterniflora |
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| Authors: | David E Padgett Courtney T Hackney Ronald K Sizemore |
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| Institution: | (1) Department of Biological Sciences, The University of North Carolina at Wilmington, 28403 Wilmington, NC, U.S.A. |
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| Abstract: | A number of antibiotics were used to suppress bacterial activity in decomposing Spartina alterniflora. The effectiveness of each treatment was quantified using INT formazan vital staining and epifluorescent microscopy. Bacterial suppression of selected treatments was verified using standard plate count procedures. Chloramphenicol treated samples (exhibiting 87–90% bacterial suppression) were analyzed respirometrically and found to consume only 30% less O2 than controls. Non-bacterial respiration (probably fungal) accounted for 70% of the respiration. |
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| Keywords: | Spartina alterniflora decomposition respiration |
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