复合脂肪酶催化生物柴油的初步研究

初步探讨了复合脂肪酶催化生物柴油的工艺。优化了复合酶配比条件和叔丁醇反应体系。在无溶剂体系中，Novozym435分别与Lipozyme TLIM和Lipozyme RMIM均以70／30质量比混合时，甲酯得率分别达到94．52％和96．25％，比Novozym435单独催化时的甲酯得率分别提高了9．52％和9．99％。在叔丁醇体系中，当Novozym435与Li-pozyme TLIM和Lipozyme RMIM分别以60／40和80／20的质量比混合时，其甲酯得率分别为85．06％和81．5％，比Novozym435单独催化的效率分别提高了9．89％和7．48％。优化叔丁醇体系中复合酶催化条件后，甲酯得率达92％。

Study on production of biodiesel with compound lipases

The biodiesel production with complex lipases was investigated. The complex lipase was higher efficiency of catalysis for transesterification than single lipase based on comparison. The ratio of two lipases of the complex was optimized, and the reaction condition in tert-butanol system was optimized, too. In solvent-free system, when Novozym435 was mixed with Lipozyme TLIM and Lipozyme RMIM at the ratio of 70/30, the methyl ester yields were higher than that of only with Novozym435 by 9.52% and 9.99% respectively. While in tert-butanol system, when Novozym435 was mixed with Lipozyme TLIM and Lipozyme RMIM at the ratio of 60/40 and 80/20 respectively, the methyl ester yields were higher than that of only with Novozym435 by 9.89% and 7.48% respectively. With the optimal reaction condition in the ten-butanol system, the methyl ester yield reached 92%.